At the end of that game, Peter’s Aunt May, the woman who raised him, died.

Source: https://www.bupipedream.com/opinions/columnists/144132/spider-man-2s-story-changed-too-much/

Aunt May barely has time to discover this revelation before agents of the criminal Silvermane arrive and launched a rocket.

Source: https://www.cbr.com/spider-man-daredevil-born-again-what-if-marvel/

During the ensuing battle, when Scorpion is about to maul Aunt May, Otto is struck by a lingering, loving memory left in Peter's body and thwarts the attack.

Source: https://collider.com/spider-man-doc-ock-body-swap-marvel-comics/

He threw Gwen Stacy off the George Washington Bridge, masterminded the Clone Saga, went after Aunt May, and tried to kill Spider-Man every chance he got.

Source: https://www.cbr.com/surprising-team-ups-marvel-comics/

The 70s Spider-Man proved that Peter was capable of on-screen superheroics with little support from others, even Aunt May in the 1977 film being less than helpful.

Source: https://screenrant.com/spider-man-4-first-live-action-lesson/